5-Keto-D-fructose production from sugar alcohol by isolated wild strain <i>Gluconobacter frateurii</i> CHM 43.

Adachi, Osao; Nguyen, Thuy M; Hours, Roque A; Kataoka, Naoya; Matsushita, Kazunobu; Akakabe, Yoshihiko; Yakushi, Toshiharu

5-Keto-D-fructose production from sugar alcohol by isolated wild strain Gluconobacter frateurii CHM 43.

Adachi, Osao; Nguyen, Thuy M; Hours, Roque A; Kataoka, Naoya; Matsushita, Kazunobu; Akakabe, Yoshihiko; Yakushi, Toshiharu.

Afiliação

Adachi O; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Japan.
Nguyen TM; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Japan.
Hours RA; CINDEFI, School of Science, La Plata National University , La Plata, Argentina.
Kataoka N; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Japan.
Matsushita K; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Japan.
Akakabe Y; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Japan.
Yakushi T; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University , Yamaguchi, Japan.

Biosci Biotechnol Biochem ; 84(8): 1745-1747, 2020 Aug.

Article em En | MEDLINE | ID: mdl-32427050

ABSTRACT

ABSTRACT

GLUCONOBACTER FRATEURII CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydrogenase) and flavoprotein D-fructose dehydrogenase in the membranes. When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield was realized with the resting cells. The method proposed here should give a smart strategy for 5-keto-D-fructose production.

Assuntos

Proteínas de Bactérias/metabolismo; Desidrogenases de Carboidrato/genética; Fermentação/genética; Frutose/análogos & derivados; Gluconobacter/enzimologia; Manitol Desidrogenases/metabolismo; Proteínas de Bactérias/genética; Desidrogenases de Carboidrato/metabolismo; Membrana Celular/enzimologia; Membrana Celular/genética; Frutose/biossíntese; Frutose/isolamento & purificação; Expressão Gênica; Gluconobacter/genética; Humanos; Concentração de Íons de Hidrogênio; Microbiologia Industrial; Manitol/metabolismo; Manitol Desidrogenases/genética; Estereoisomerismo

Palavras-chave

Gluconobacter frateurii; 5-keto-D-fructose; Acetic acid bacteria; oxidative fermentation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Desidrogenases de Carboidrato / Gluconobacter / Fermentação / Frutose / Manitol Desidrogenases Limite: Humans Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Japão

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