instaGRAAL: chromosome-level quality scaffolding of genomes using a proximity ligation-based scaffolder.
Genome Biol
; 21(1): 148, 2020 06 18.
Article
em En
| MEDLINE
| ID: mdl-32552806
ABSTRACT
Hi-C exploits contact frequencies between pairs of loci to bridge and order contigs during genome assembly, resulting in chromosome-level assemblies. Because few robust programs are available for this type of data, we developed instaGRAAL, a complete overhaul of the GRAAL program, which has adapted the latter to allow efficient assembly of large genomes. instaGRAAL features a number of improvements over GRAAL, including a modular correction approach that optionally integrates independent data. We validate the program using data for two brown algae, and human, to generate near-complete assemblies with minimal human intervention.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alga Marinha
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Software
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Cromossomos
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Genômica
Limite:
Humans
Idioma:
En
Revista:
Genome Biol
Assunto da revista:
BIOLOGIA MOLECULAR
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GENETICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
França