Toxicity of soil antimony to earthworm Eisenia fetida (Savingy) before and after the aging process.

ABSTRACT

Information on soil antimony (Sb) toxicity to earthworm Eisenia fetida (Savingy) is limited. This ecotoxicology study was designed to quantify the soil Sb toxicity to earthworm E. fetida before and after aging process, establishing dose-effect relationship between Sb content and mortality. Results of the avoidance test and acute test showed that the values of net avoidance response, escape rate and mortality were generally decreased in aged treatment compared to that in fresh treatment, respectively from 93.33% to 66.67%, 36.67% to 13.33% and 100% to 53.33% (15 d) taking TL800 (treatment level of 800 mg/kg) for example, meanwhile the values of median lethal content (LC50) at 72 h, 7 d and 15 d were respectively increased from 355.27 mg/kg to 2324.55 mg/kg, 322.19 mg/kg and 1743.19 mg/kg and 282.74 mg/kg to 745.94 mg/kg, indicating that aging process could reduce the Sb acute toxicity to earthworm. According to a three-step sequential extraction procedure, the bioavailable Sb ranged from 24.45% to 43.24% and 16.97% to 27.70% in fresh treatment and aged treatment, respectively, and the mortality of earthworm for 24 h decreased with the decrease of the content of mild acid-soluble antimony (which decreased averagely from 23.09% to 14.00%), which was more suited to assess Sb toxicity. This is the first report that confirms the toxicity of soil Sb to earthworm E. fetida as well as the considering of aging process and speciation.