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Evaluation of stemness marker expression in bovine ovarian granulosa cells.
Hoang, Son Nghia; Ho, Chi Nguyen Quynh; Nguyen, Thao Thi Phuong; Doan, Chung Chinh; Tran, Diem Hong; Le, Long Thanh.
Afiliação
  • Hoang SN; Animal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Ho CNQ; Animal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Nguyen TTP; Animal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Doan CC; Animal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Tran DH; Department of Internal Medicine - Cardiology, UT Southwestern Medical Center, Dallas, TX.
  • Le LT; Animal Biotechnology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
Anim Reprod ; 16(2): 277-281, 2019 Oct 23.
Article em En | MEDLINE | ID: mdl-33224287
ABSTRACT
The objective of this study was to assess the stemness marker expressions (Oct4, Nanog, and Sox2) of granulosa cells (GCs) collected from bovine ovarian follicles and in vitro expansion. The single bovine ovarian follicles were isolated and categorized into 4 groups according to their diameter including group A (<2 mm), group B (2-3 mm), group C (3-4 mm), and group D (>4 mm). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and immunostaining were applied to evaluate the stemness marker expression of bovine GCs from ovarian follicles. We also estimated the stemness marker transcript expressions of GCs during in vitro expression by qRT-PCR. qRT-PCR analysis demonstrated that fresh GCs from bovine ovarian follicles expressed the stemness markers (Oct4, Nanog, Sox2). These markers were down-regulated during antral stage follicular development. We also estimated stemness marker transcript expressions of GCs which were isolated and in vitro expanded from ovarian follicles of group A. The qRT-PCR results showed that Oct4 and Sox2 transcript expressions were reduced during in vitro expansion while Nanog transcript was not expressed.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Anim Reprod Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Vietnã

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Anim Reprod Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Vietnã