U2AF - Hypoxia-induced fas alternative splicing regulator.
Exp Cell Res
; 399(1): 112444, 2021 02 01.
Article
em En
| MEDLINE
| ID: mdl-33347855
ABSTRACT
The splicing machinery heavily contributes to biological complexity and especially to the ability of cells to adapt to altered cellular conditions. Hypoxia also plays a key role in the pathophysiology of many disease states. Recent studies have revealed that tumorigenesis and hypoxia are involved in large-scale alterations in alternative pre-mRNA splicing. Fas pre-mRNA is alternatively spliced by excluding exon 6 to produce soluble Fas (sFas) protein that lacks a transmembrane domain and acts by inhibiting Fas mediated apoptosis. In the present study we show that U2AF is involved in hypoxia dependent anti-apoptotic Fas mRNA isoform formation. Our performed studies show that U2AF-RNA interaction is reduced in hypoxic cells, leading to reduction of Fas and increased sFas mRNAs formation. Efficient U2AF-RNA interactions of both subunits are important for Fas exon 6 inclusion into forming mRNA in normoxic and hypoxic cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptor fas
/
Fator de Processamento U2AF
/
Hipóxia
Limite:
Humans
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Lituânia