SCC: an accurate imputation method for scRNA-seq dropouts based on a mixture model.
BMC Bioinformatics
; 22(1): 5, 2021 Jan 06.
Article
em En
| MEDLINE
| ID: mdl-33407064
ABSTRACT
BACKGROUND:
Single-cell RNA sequencing (scRNA-seq) enables the possibility of many in-depth transcriptomic analyses at a single-cell resolution. It's already widely used for exploring the dynamic development process of life, studying the gene regulation mechanism, and discovering new cell types. However, the low RNA capture rate, which cause highly sparse expression with dropout, makes it difficult to do downstream analyses.RESULTS:
We propose a new method SCC to impute the dropouts of scRNA-seq data. Experiment results show that SCC gives competitive results compared to two existing methods while showing superiority in reducing the intra-class distance of cells and improving the clustering accuracy in both simulation and real data.CONCLUSIONS:
SCC is an effective tool to resolve the dropout noise in scRNA-seq data. The code is freely accessible at https//github.com/nwpuzhengyan/SCC .Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Citoplasmático Pequeno
/
Perfilação da Expressão Gênica
/
Análise de Célula Única
Idioma:
En
Revista:
BMC Bioinformatics
Assunto da revista:
INFORMATICA MEDICA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China