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Less polar ginsenosides have better protective effects on mice infected by Listeria monocytogenes.
Jing, Jinjin; Zhang, Ruoyu; Wang, Yunhai; Tang, Shaojian; Yang, Hanchao; Du, Lidong; Lin, Bingjie; Shao, Lijun; Zhang, Fengxiang; Xue, Peng.
Afiliação
  • Jing J; School of Public Health, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Zhang R; School of Public Health, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Wang Y; Affiliated Hospital of Weifang Medical University, Weifang 261053, People's Republic of China.
  • Tang S; School of Pharmacy, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Yang H; Affiliated Hospital of Weifang Medical University, Weifang 261053, People's Republic of China.
  • Du L; Clinical Medical Colleges, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Lin B; School of Public Health, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Shao L; School of Public Health, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Zhang F; School of Public Health, Weifang Medical University, Weifang 261053, People's Republic of China.
  • Xue P; School of Public Health, Weifang Medical University, Weifang 261053, People's Republic of China. Electronic address: jplxp26@126.com.
Ecotoxicol Environ Saf ; 213: 112065, 2021 Apr 15.
Article em En | MEDLINE | ID: mdl-33636464
ABSTRACT
Listeria monocytogenes widely exists in the natural environment and does great harm, which can cause worldwide public safety problem. Infection with L. monocytogenes can cause rapid death of Kupffer cell (KCs) in liver tissue and liver damage. American ginseng saponins is a natural compound in plants, which has great potential in inhibiting L. monocytogenes infection. Therefore, American ginseng stem-leaf saponins (AGS) and American ginseng heat-transformed saponins (HTS) were used as raw materials to study their bacteriostatic experiments in vivo and in vitro. In this experiment, female Kunming mice were randomly divided into five groups control group, negative group, AGS group, HTS group (10 mg/kg/day in an equal volume via gastric administration) and penicillin group, each group containing six mice. Profiles AGS and HTS components were evaluated by high-performance liquid chromatography (HPLC) analysis. The bacteriostatic effect of AGS and HTS on L. monocytogenes was evaluated by inhibition zone test, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The bacteriostatic effect of AGS and HTS pretreatment on mice infected with L. monocytogenes were studies by animal experimental. The results showed that the content of polar saponins in AGS was 0.81 ± 0.003 mg/mg, less polar saponins was 0.08 ± 0.02 mg/mg, the content of polar saponins in HTS was 0.10 ± 0.01 mg/mg, less polar saponins was 0.76 ± 0.02 mg/mg. The in vitro bacteriostatic diameter of HTS (16.6 ± 0.8 mm) is large than that of AGS (10.2 ± 1.2 mm). AGS and HTS pretreatment could reduce the colony numbers in the livers of mice infected with Listeria monocytogenes. The levels of alanine aminotransferase (ALT), IL-1ß, IL-6, TNF-α and IFN-γ in the livers of mice in the pretreatment group were significantly lower than those in the negative group. There were obvious leukoplakia, calcification and other liver damage on the liver surface in the negative control group, and obvious inflammatory cell infiltration in HE sections. AGS and HTS pretreatment can reduce liver injury caused by L. monocytogenes and protect the liver. Compared with AGS, HTS has higher content of less polar saponins and better bacteriostatic effect in vitro. The count of bacterial in liver tissue of HTS group was significantly lower, the survival rate was significantly higher than that of AGS group. Less polar saponins had better bacteriostatic effect. Collectively, less polar saponins pretreatment has a protective effect on mice infected with L. monocytogenes, to which alleviated liver damage, improved anti-inflammatory ability and immunity of the body, protected liver may contribute.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ginsenosídeos / Listeria monocytogenes Limite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ginsenosídeos / Listeria monocytogenes Limite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2021 Tipo de documento: Article