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Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: a guide and case study in setting up an emergency-use, laboratory-developed molecular microbiological assay.
Anahtar, Melis N; Shaw, Bennett M; Slater, Damien; Byrne, Elizabeth H; Botti-Lodovico, Yolanda; Adams, Gordon; Schaffner, Stephen F; Eversley, Jacqueline; McGrath, Graham E G; Gogakos, Tasos; Lennerz, Jochen; Marble, Hetal Desai; Ritterhouse, Lauren L; Batten, Julie M; Georgantas, N Zeke; Pellerin, Rebecca; Signorelli, Sylvia; Thierauf, Julia; Kemball, Molly; Happi, Christian; Grant, Donald S; Ndiaye, Daouda; Siddle, Katherine J; Mehta, Samar B; Harris, Jason B; Ryan, Edward T; Pierce, Virginia M; LaRocque, Regina C; Lemieux, Jacob E; Sabeti, Pardis C; Rosenberg, Eric S; Branda, John A; Turbett, Sarah E.
Afiliação
  • Anahtar MN; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Shaw BM; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Slater D; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Byrne EH; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Botti-Lodovico Y; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Adams G; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Schaffner SF; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Eversley J; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • McGrath GEG; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Gogakos T; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Lennerz J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Marble HD; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Ritterhouse LL; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Batten JM; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Georgantas NZ; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Pellerin R; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Signorelli S; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Thierauf J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Kemball M; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Happi C; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Grant DS; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Ndiaye D; Department of Otorhinolaryngology, University Hospital Heidelberg, Heidelberg, Baden-Württemberg, Germany.
  • Siddle KJ; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Mehta SB; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Harris JB; Department of Biological Sciences, Redeemer's University, Ede, Osun, Nigeria.
  • Ryan ET; African Center of Excellence for Genomics of Infectious Diseases, Redeemer's University, Ede, Osun, Nigeria.
  • Pierce VM; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone.
  • LaRocque RC; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone.
  • Lemieux JE; African Center of Excellence for Genomics of Infectious Diseases, Redeemer's University, Ede, Osun, Nigeria.
  • Sabeti PC; Department of Mycology and Pharmacology, Universite Cheikh Anta Diop, Dakar, Senegal.
  • Rosenberg ES; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
  • Branda JA; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Turbett SE; Infectious Disease and Microbiome Program, Eli and Edythe L. Broad Institute of Harvard and MIT, Cambridge, Massachusetts, USA.
J Clin Pathol ; 74(8): 496-503, 2021 Aug.
Article em En | MEDLINE | ID: mdl-34049977
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Laboratórios Hospitalares / Serviço Hospitalar de Emergência / Reação em Cadeia da Polimerase em Tempo Real / Teste de Ácido Nucleico para COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudo: Diagnostic_studies / Prognostic_studies / Qualitative_research Limite: Humans Idioma: En Revista: J Clin Pathol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Laboratórios Hospitalares / Serviço Hospitalar de Emergência / Reação em Cadeia da Polimerase em Tempo Real / Teste de Ácido Nucleico para COVID-19 / SARS-CoV-2 / COVID-19 Tipo de estudo: Diagnostic_studies / Prognostic_studies / Qualitative_research Limite: Humans Idioma: En Revista: J Clin Pathol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos