Genetically engineered birds; pre-CRISPR and CRISPR era.
Biol Reprod
; 106(1): 24-46, 2022 01 13.
Article
em En
| MEDLINE
| ID: mdl-34668968
ABSTRACT
Generating biopharmaceuticals in genetically engineered bioreactors continues to reign supreme. Hence, genetically engineered birds have attracted considerable attention from the biopharmaceutical industry. Fairly recent genome engineering methods have made genome manipulation an easy and affordable task. In this review, we first provide a broad overview of the approaches and main impediments ahead of generating efficient and reliable genetically engineered birds, and various factors that affect the fate of a transgene. This section provides an essential background for the rest of the review, in which we discuss and compare different genome manipulation methods in the pre-clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR era in the field of avian genome engineering.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aves
/
Engenharia Genética
/
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Limite:
Animals
Idioma:
En
Revista:
Biol Reprod
Ano de publicação:
2022
Tipo de documento:
Article