Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria.

Wang, Xue; Zheng, Wentao; Zhou, Haibo; Tu, Qiang; Tang, Ya-Jie; Stewart, A Francis; Zhang, Youming; Bian, Xiaoying

Wang, Xue; Zheng, Wentao; Zhou, Haibo; Tu, Qiang; Tang, Ya-Jie; Stewart, A Francis; Zhang, Youming; Bian, Xiaoying.

Afiliação

Wang X; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Zheng W; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Zhou H; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Tu Q; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Tang YJ; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Stewart AF; Genomics, Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Tatzberg 47-51, 01307 Dresden, Germany.
Zhang Y; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Bian X; Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.

Nucleic Acids Res ; 50(3): e15, 2022 02 22.

Article em En | MEDLINE | ID: mdl-34792175

Assuntos

DNA; Engenharia Genética; Bactérias/genética; DNA de Cadeia Simples/genética; Genoma Bacteriano/genética; Oligonucleotídeos/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Engenharia Genética Idioma: En Revista: Nucleic Acids Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

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