Production of pentaglycine-fused proteins using Escherichia coli expression system without in vitro peptidase treatment.

Sakono, Masafumi; Oshima, Tatsuki; Iwakawa, Takako; Arai, Ryoichi

Sakono, Masafumi; Oshima, Tatsuki; Iwakawa, Takako; Arai, Ryoichi.

Afiliação

Sakono M; Department of Applied Chemistry, Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan. Electronic address: msakono@eng.u-toyama.ac.jp.
Oshima T; Department of Applied Chemistry, Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan.
Iwakawa T; Department of Applied Chemistry, Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan.
Arai R; Department of Biomolecular Innovation, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Ueda, Nagano, 386-8567, Japan; Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japa

Protein Expr Purif ; 194: 106068, 2022 06.

Article em En | MEDLINE | ID: mdl-35158072

Assuntos

Escherichia coli; Peptídeo Hidrolases; Sequência de Aminoácidos; Códon/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas de Fluorescência Verde/metabolismo; Peptídeo Hidrolases/metabolismo

Palavras-chave

Methionyl aminopeptidase; Peptide ligation; Polyglycine; Protein expression; Sortase A

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeo Hidrolases / Escherichia coli Idioma: En Revista: Protein Expr Purif Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article

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