Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated pheS for Counterselection.

Ran Sapir, Shai; Boichis, Etai; Herskovits, Anat A

Ran Sapir, Shai; Boichis, Etai; Herskovits, Anat A.

Afiliação

Ran Sapir S; The Shmunis School of Biomedicine and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
Boichis E; The Shmunis School of Biomedicine and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
Herskovits AA; The Shmunis School of Biomedicine and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel. anathe@tauex.tau.ac.il.

Methods Mol Biol ; 2427: 3-10, 2022.

Article em En | MEDLINE | ID: mdl-35619020

Assuntos

Listeria monocytogenes; Alelos; Deleção de Genes; Listeria monocytogenes/genética; Mutação; Fenilalanina/genética

Palavras-chave

Allelic exchange; Counterselection; Gibson assembly; Listeria monocytogenes; Mutagenesis; pheS

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Listeria monocytogenes Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Israel

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