Genetic variants associated with two major bovine milk fatty acids offer opportunities to breed for altered milk fat composition.
Genet Sel Evol
; 54(1): 35, 2022 May 26.
Article
em En
| MEDLINE
| ID: mdl-35619070
ABSTRACT
BACKGROUND:
Although bovine milk is regarded as healthy and nutritious, its high content of saturated fatty acids (FA) may be harmful to cardiovascular health. Palmitic acid (C160) is the predominant saturated FA in milk with adverse health effects that could be countered by substituting it with higher levels of unsaturated FA, such as oleic acid (C181cis-9). In this work, we performed genome-wide association analyses for milk fatty acids predicted from FTIR spectroscopy data using 1811 Norwegian Red cattle genotyped and imputed to a high-density 777k single nucleotide polymorphism (SNP)-array. In a follow-up analysis, we used imputed whole-genome sequence data to detect genetic variants that are involved in FTIR-predicted levels of C160 and C181cis-9 and explore the transcript profile and protein level of candidate genes.RESULTS:
Genome-wise significant associations were detected for C160 on Bos taurus (BTA) autosomes 11, 16 and 27, and for C181cis-9 on BTA5, 13 and 19. Closer examination of a significant locus on BTA11 identified the PAEP gene, which encodes the milk protein ß-lactoglobulin, as a particularly attractive positional candidate gene. At this locus, we discovered a tightly linked cluster of genetic variants in coding and regulatory sequences that have opposing effects on the levels of C160 and C181cis-9. The favourable haplotype, linked to reduced levels of C160 and increased levels of C181cis-9 was also associated with a marked reduction in PAEP expression and ß-lactoglobulin protein levels. ß-lactoglobulin is the most abundant whey protein in milk and lower levels are associated with important dairy production parameters such as improved cheese yield.CONCLUSIONS:
The genetic variants detected in this study may be used in breeding to produce milk with an improved FA health-profile and enhanced cheese-making properties.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estudo de Associação Genômica Ampla
/
Ácidos Graxos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Genet Sel Evol
Assunto da revista:
BIOLOGIA
/
GENETICA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Noruega