A multiplexed, three-dimensional pooling and next-generation sequencing strategy for creating barcoded mutant arrays: construction of a Schizosaccharomyces pombe transposon insertion library.

Li, Yanhui; Molyneaux, Neil; Zhang, Haitao; Zhou, Gang; Kerr, Carly; Adams, Mark D; Berkner, Kathleen L; Runge, Kurt W

Li, Yanhui; Molyneaux, Neil; Zhang, Haitao; Zhou, Gang; Kerr, Carly; Adams, Mark D; Berkner, Kathleen L; Runge, Kurt W.

Afiliação

Li Y; Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH 44195, USA.
Molyneaux N; Department of Genetics and Genomic Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Zhang H; Department of Genetics and Genomic Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Zhou G; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH 44195, USA.
Kerr C; Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH 44195, USA.
Adams MD; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH 44195, USA.
Berkner KL; Department of Genetics and Genomic Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Runge KW; Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic Lerner College of Medicine at Case Western Reserve University, Cleveland, OH 44195, USA.

Nucleic Acids Res ; 50(17): e102, 2022 09 23.

Article em En | MEDLINE | ID: mdl-35766443

Assuntos

Schizosaccharomyces; DNA; Elementos de DNA Transponíveis/genética; Biblioteca Gênica; Genes Essenciais; Sequenciamento de Nucleotídeos em Larga Escala/métodos; Mutagênese Insercional; RNA não Traduzido; Saccharomyces cerevisiae/genética; Schizosaccharomyces/genética; Regiões não Traduzidas

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schizosaccharomyces Idioma: En Revista: Nucleic Acids Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

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