Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery.
J Cell Biol
; 221(12)2022 12 05.
Article
em En
| MEDLINE
| ID: mdl-36156058
ABSTRACT
The mating of budding yeast depends on chemotropism, a fundamental cellular process. Haploid yeast cells of opposite mating type signal their positions to one another through mating pheromones. We have proposed a deterministic gradient sensing model that explains how these cells orient toward their mating partners. Using the cell-cycle determined default polarity site (DS), cells assemble a gradient tracking machine (GTM) composed of signaling, polarity, and trafficking proteins. After assembly, the GTM redistributes up the gradient, aligns with the pheromone source, and triggers polarized growth toward the partner. Since positive feedback mechanisms drive polarized growth at the DS, it is unclear how the GTM is released for tracking. What prevents the GTM from triggering polarized growth at the DS? Here, we describe two mechanisms that are essential for tracking inactivation of the Ras GTPase Bud1 and positioning of actin-independent vesicle delivery upgradient.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas rab de Ligação ao GTP
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Vesículas Transportadoras
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Israel