Characterization of a polysaccharide from Polygala tenuifolia willd. with immune activity via activation MAPKs pathway.
Bioorg Chem
; 130: 106214, 2023 01.
Article
em En
| MEDLINE
| ID: mdl-36332314
ABSTRACT
Polysaccharides from the Polygala tenuifolia Willd. have been shown multiple biological activities, however the structural feature and immunomodulatory activity are still rarely reported. In this study, a polysaccharide was obtained by purification, and its structural characteristics and immune activity were analyzed. The polysaccharide was a homogeneous macromolecular polysaccharide with smooth flat flakes surface structure and molecular weight of 2.34 × 105 Da, and composed of Rha, Ara, Xyl, Man, Glc, Gal. Methylation and NMR analyses confirmed that the repeating unit of polysaccharide was [â3)-α-Araf-(1 â 3)-α-Araf-(1 â 5)-α-Araf-(1 â 5)-α-Araf-(1 â 3)-α-Araf-(1 â ]n, and the side chain was α-Araf-(1 â 6)-ß-Galp-(1 â 6)-ß-Glcp-(1 â 6)-α-Manp-(1â, which was attached to the C3 of â 3,5)-α-Araf-(1 â. In vitro, the RAW 264.7 cells were co-cultivated with LPS and polysaccharide, and the results revealed that the polysaccharide can promote cell proliferation, activate effectors to release cytokines (TNF-α, IL-6, IL-1ß), and then activate macrophages for immune activity. Therefore, we can infer that the polysaccharide might regard as a potential immunomodulator.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polygala
Limite:
Humans
Idioma:
En
Revista:
Bioorg Chem
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China