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Multi-micron crisscross structures grown from DNA-origami slats.
Wintersinger, Christopher M; Minev, Dionis; Ershova, Anastasia; Sasaki, Hiroshi M; Gowri, Gokul; Berengut, Jonathan F; Corea-Dilbert, F Eduardo; Yin, Peng; Shih, William M.
Afiliação
  • Wintersinger CM; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Minev D; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA.
  • Ershova A; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
  • Sasaki HM; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
  • Gowri G; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Berengut JF; John A. Paulson School of Engineering and Applied Sciences, Harvard University, Boston, MA, USA.
  • Corea-Dilbert FE; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
  • Yin P; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
  • Shih WM; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Nat Nanotechnol ; 18(3): 281-289, 2023 03.
Article em En | MEDLINE | ID: mdl-36543881
ABSTRACT
Living systems achieve robust self-assembly across a wide range of length scales. In the synthetic realm, nanofabrication strategies such as DNA origami have enabled robust self-assembly of submicron-scale shapes from a multitude of single-stranded components. To achieve greater complexity, subsequent hierarchical joining of origami can be pursued. However, erroneous and missing linkages restrict the number of unique origami that can be practically combined into a single design. Here we extend crisscross polymerization, a strategy previously demonstrated with single-stranded components, to DNA-origami 'slats' for fabrication of custom multi-micron shapes with user-defined nanoscale surface patterning. Using a library of ~2,000 strands that are combinatorially arranged to create unique DNA-origami slats, we realize finite structures composed of >1,000 uniquely addressable slats, with a mass exceeding 5 GDa, lateral dimensions of roughly 2 µm and a multitude of periodic structures. Robust production of target crisscross structures is enabled through strict control over initiation, rapid growth and minimal premature termination, and highly orthogonal binding specificities. Thus crisscross growth provides a route for prototyping and scalable production of structures integrating thousands of unique components (that is, origami slats) that each is sophisticated and molecularly precise.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanotecnologia / Nanoestruturas Idioma: En Revista: Nat Nanotechnol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanotecnologia / Nanoestruturas Idioma: En Revista: Nat Nanotechnol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos