Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster.

Venken, Koen J T; Matinyan, Nick; Gonzalez, Yezabel; Sarrion-Perdigones, Alejandro; Dierick, Herman A

Venken, Koen J T; Matinyan, Nick; Gonzalez, Yezabel; Sarrion-Perdigones, Alejandro; Dierick, Herman A.

Afiliação

Venken KJT; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.
Matinyan N; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas.
Gonzalez Y; Integrative Molecular Biomedical Sciences Graduate Program, Baylor College of Medicine, Houston, Texas.
Sarrion-Perdigones A; McNair Medical Institute at The Robert and Janice McNair Foundation, Baylor College of Medicine, Houston, Texas.
Dierick HA; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas.

Curr Protoc ; 3(2): e653, 2023 Feb.

Article em En | MEDLINE | ID: mdl-36757602

Assuntos

DNA; Drosophila melanogaster; Animais; Drosophila melanogaster/genética; Clonagem Molecular; Animais Geneticamente Modificados/genética; Plasmídeos/genética

Palavras-chave

Drosophila melanogaster; GoldenBraid 2.0; counterselection; gene expression analysis; multiplexed; selection; synthetic assembly cloning; transgenesis

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Drosophila melanogaster Limite: Animals Idioma: En Revista: Curr Protoc Ano de publicação: 2023 Tipo de documento: Article

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