Thorough research and modification of one-carbon units cycle for improving L-methionine production in Escherichia coli.
3 Biotech
; 13(6): 203, 2023 Jun.
Article
em En
| MEDLINE
| ID: mdl-37220602
ABSTRACT
Methionine is the only one of the essential amino acids that contain sulfur, widely used as a feed additive in agriculture. In this study, the availability of 5-methyl-tetrahydrofolate was confirmed as the main limitation in the complex multibranched biosynthetic pathway of L-methionine. The cycle of one-carbon units was thoroughly investigated and modified to supply 5-methyl-tetrahydrofolate for L-methionine production, such as enhancing the supply of precursor, expediting the conversion rate of the cycle, introducing exogenous serine hydroxymethyltransferase and increasing pool size of one-carbon units carrier. The final strain MYA/pAmFA-4 was able to produce 20.89 g/L L-methionine by fed-batch fermentation, which was the highest titer reported in the literatures. This study is instructive for other metabolites biosynthesized needing one-carbon units or having a complex multibranched biosynthetic pathway. Supplementary Information The online version contains supplementary material available at 10.1007/s13205-023-03625-9.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
3 Biotech
Ano de publicação:
2023
Tipo de documento:
Article