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Prokaryotic expression of the V protein of the peste des petits ruminants virus and development of an indirect ELISA.
Lu, Guili; Wang, Ping; Miao, Shukui; Huang, Jiong; Ma, Wenge; Mi, Xiaoyun; Xue, Jing; Shayilan, Kayizha; Yang, Xueyun; Yan, Genqiang.
Afiliação
  • Lu G; College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, P. R. China.
  • Wang P; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Miao S; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Huang J; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Ma W; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Mi X; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Xue J; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Shayilan K; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Yang X; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
  • Yan G; The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
Anim Biotechnol ; 34(9): 5011-5015, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37288766
ABSTRACT
In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Cabras / Vírus da Peste dos Pequenos Ruminantes / Peste dos Pequenos Ruminantes Limite: Animals Idioma: En Revista: Anim Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2023 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Cabras / Vírus da Peste dos Pequenos Ruminantes / Peste dos Pequenos Ruminantes Limite: Animals Idioma: En Revista: Anim Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2023 Tipo de documento: Article