Bdellovibrio predation cycle characterized at nanometre-scale resolution with cryo-electron tomography.
Nat Microbiol
; 8(7): 1267-1279, 2023 07.
Article
em En
| MEDLINE
| ID: mdl-37349588
ABSTRACT
Bdellovibrio bacteriovorus is a microbial predator that offers promise as a living antibiotic for its ability to kill Gram-negative bacteria, including human pathogens. Even after six decades of study, fundamental details of its predation cycle remain mysterious. Here we used cryo-electron tomography to comprehensively image the lifecycle of B. bacteriovorus at nanometre-scale resolution. With high-resolution images of predation in a native (hydrated, unstained) state, we discover several surprising features of the process, including macromolecular complexes involved in prey attachment/invasion and a flexible portal structure lining a hole in the prey peptidoglycan that tightly seals the prey outer membrane around the predator during entry. Unexpectedly, we find that B. bacteriovorus does not shed its flagellum during invasion, but rather resorbs it into its periplasm for degradation. Finally, following growth and division in the bdelloplast, we observe a transient and extensive ribosomal lattice on the condensed B. bacteriovorus nucleoid.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bdellovibrio
/
Bdellovibrio bacteriovorus
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Microbiol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos