Your browser doesn't support javascript.
loading
Brown adipocyte activation mediates lipid metabolism through exosomal tRNA-derived fragments.
Peng, Zhou; Cai, Jingyang; Guo, Xirong; Xu, Siliang.
Afiliação
  • Peng Z; Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Cai J; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
  • Guo X; Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: xrguo@shsmu.edu.cn.
  • Xu S; State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, China. Electronic address: siliangxu2015@njmu.edu.cn.
Biochem Biophys Res Commun ; 672: 128-136, 2023 09 10.
Article em En | MEDLINE | ID: mdl-37352601
ABSTRACT
Human obesity is related with intrinsic impairments of adipocyte lipolysis and ectopic lipid accumulation. Small regulatory RNAs, such as tRNA-derived fragments (tRFs) and tRNA halves (tiRNAs), are enriched in exosomes and play a crucial role in lipid metabolism. To determine certain tRFs for lipolysis, brown adipocytes were treated with forskolin. Using tRFs sequencing, 207 different expressed exosomal tRFs were determined. In forskolin samples, 145 downregulated and 62 upregulated tRFs were identified. Further, qRT-PCR validated that three notably upregulated tRFs (tRF-Gly-GCC-007, tRF-Gly-GCC-008, and tRF-Gly-GCC-009) were in accordance with the sequencing result. Target genes of tRFs were involved in positive regulation of protein phosphorylation and cell adhesion process by significantly downregulating UCHL1 expression, which might participate in lipolysis. This study might provide therapeutic targets and potential diagnostic biomarkers for obesity treatment.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metabolismo dos Lipídeos / Adipócitos Marrons Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metabolismo dos Lipídeos / Adipócitos Marrons Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China