Multiplex Single-Nucleotide Microbial Genome Editing Achieved by CRISPR-Cas9 Using 5'-End-Truncated sgRNAs.

Lim, Se Ra; Lee, Ho Joung; Kim, Hyun Ju; Lee, Sang Jun

Lim, Se Ra; Lee, Ho Joung; Kim, Hyun Ju; Lee, Sang Jun.

Afiliação

Lim SR; Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea.
Lee HJ; Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea.
Kim HJ; Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea.
Lee SJ; Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University, Anseong 17546, Republic of Korea.

ACS Synth Biol ; 12(7): 2203-2207, 2023 07 21.

Article em En | MEDLINE | ID: mdl-37368988

Assuntos

Edição de Genes; RNA Guia de Sistemas CRISPR-Cas; Edição de Genes/métodos; Sistemas CRISPR-Cas/genética; Nucleotídeos; Escherichia coli/genética; Genoma Microbiano

Palavras-chave

CRISPR-Cas; multiplex; single-nucleotide editing; truncated sgRNA

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Edição de Genes / RNA Guia de Sistemas CRISPR-Cas Idioma: En Revista: ACS Synth Biol Ano de publicação: 2023 Tipo de documento: Article

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