Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability.
Stem Cells
; 41(10): 907-915, 2023 Oct 08.
Article
em En
| MEDLINE
| ID: mdl-37386941
ABSTRACT
The role of serum response factor (Srf), a central mediator of actin dynamics and mechanical signaling, in cell identity regulation is debated to be either a stabilizer or a destabilizer. We investigated the role of Srf in cell fate stability using mouse pluripotent stem cells. Despite the fact that serum-containing cultures yield heterogeneous gene expression, deletion of Srf in mouse pluripotent stem cells leads to further exacerbated cell state heterogeneity. The exaggerated heterogeneity is detectible not only as increased lineage priming but also as the developmentally earlier 2C-like cell state. Thus, pluripotent cells explore more variety of cellular states in both directions of development surrounding naïve pluripotency, a behavior that is constrained by Srf. These results support that Srf functions as a cell state stabilizer, providing rationale for its functional modulation in cell fate intervention and engineering.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator de Resposta Sérica
/
Células-Tronco Pluripotentes
Limite:
Animals
Idioma:
En
Revista:
Stem Cells
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos