Redox Properties and in Vivo Magnetic Resonance Imaging of Cyclodextrin-Polynitroxides Contrast Agents.
Chemphyschem
; 24(19): e202300100, 2023 10 04.
Article
em En
| MEDLINE
| ID: mdl-37431722
ABSTRACT
This paper reports the synthesis, characterization and inâ
vivo application of water-soluble supramolecular contrast agents (Mw 5-5.6â
kDa) for MRI obtained from ß-cyclodextrin functionalized with different kinds of nitroxide radicals, both with piperidine structure (CD2 and CD3) and with pyrrolidine structure (CD4 and CD5). As to the stability of the radicals in presence of ascorbic acid, CD4 and CD5 have low second order kinetic constants (≤0.05â
M-1 s-1 ) compared to CD2 (3.5â
M-1 s-1 ) and CD3 (0.73â
M-1 s-1 ). Relaxivity (r1 ) measurements on compounds CD3-CD5 were carried out at different magnetic field strength (0.7, 3, 7 and 9.4 T). At 0.7â
T, r1 values comprised between 1.5â
mM-1 s-1 and 1.9â
mM-1 s-1 were found while a significant reduction was observed at higher fields (r1 ≈0.6-0.9â
mM-1 s-1 at 9.4 T). Tests inâ
vitro on HEK293 human embryonic kidney cells, L929 mouse fibroblasts and U87 glioblastoma cells indicated that all compounds were non-cytotoxic at concentrations below 1â
µmol mL-1 . MRI inâ
vivo was carried out at 9.4â
T on glioma-bearing rats using the compounds CD3-CD5. The experiments showed a good lowering of T1 relaxation in tumor with a retention of the contrast for at least 60 mins confirming improved stability also inâ
vivo conditions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Meios de Contraste
/
Ciclodextrinas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Chemphyschem
Assunto da revista:
BIOFISICA
/
QUIMICA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Itália