Kurloff cell proteoglycans. Evidence for de novo synthesis of chondroitin sulphate proteoglycans by purified Kurloff cells.
FEBS Lett
; 209(2): 299-304, 1986 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-3792549
ABSTRACT
This paper reports the first direct demonstration of de novo synthesis of chondroitin sulphate proteoglycans by Kurloff cells. This was achieved using highly purified splenic Kurloff cells labelled in vitro with [35S]sulphate and D-[U-3H]glucosamine. A single population of sulphated proteoglycans was observed after dissociative extraction, DEAE-cellulose chromatography, Sepharose CL 6B chromatography and fluorography after electrophoresis. These were large, highly anionic proteoglycans and were completely digested by chondroitinase AC or ABC. Moreover, glycosaminoglycan extracted from Kurloff cells had the electrophoretic mobility of control chondroitin sulphate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas de Sulfatos de Condroitina
/
Proteoglicanas
/
Baço
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1986
Tipo de documento:
Article