Effects of hachimijiogan, tokishakuyakusan and keishibukuryogan on estrogen and progesterone secretions by rat preovulatory follicles.

ABSTRACT

Ovaries of rats 48 hours after injection of 10 IU PMS when 27 days old were incubated or perifused with NIH-LH, Hachimijiogan (TJ-7), Tokishakuyakusan (TJ-23) and Keishibukuryogan (TJ-25). TJ-7, TJ-23 and TJ-25 decreased estradiol-17 beta (E2) levels in media and LH effects on progesterone secretions, while they increased progesterone in media and LH effects on E2 secretions. These results suggest that TJ-7, TJ-23 or TJ-25 stimulates preovulatory follicles to secrete progesterone but to suppress E2 secretions. Furthermore, it is indicated that their combination treatment with LH multiplies the sole effect of LH on E2 secretions but suppresses LH effects on progesterone.