[Gly14]-humanin exerts a protective effect against D-galactose-induced primary ovarian insufficiency in mice.
Reprod Biomed Online
; 48(2): 103330, 2024 02.
Article
em En
| MEDLINE
| ID: mdl-38163419
ABSTRACT
RESEARCH QUESTION Is there a protective effect of the humanin derivative [Gly14]-humanin (HNG) on a D-gal-induced mouse model of primary ovarian insufficiency (POI), and what is the underlying mechanism? DESIGN:
D-gal (200 mg/kg/day) was injected subcutaneously for 6 weeks to induce the mouse POI model. Mice treated with HNG were injected intraperitoneally with different concentrations for 6 weeks. Ovarian morphology, function, levels of sex hormones and states of oxidative stress in the ovary and body were evaluated.RESULTS:
Compared with the D-gal group, 10 mg/kg HNG improved the abnormal ovarian morphology and oestrous cycle (Pâ¯=â¯0.0036), increased the number of ovarian follicles (Pâ¯=â¯0.0016) and litters (Pâ¯=â¯0.0127), and increased the levels of oestrogen (Pâ¯=â¯0.0043) and AMH (Pâ¯=â¯0.0147). Antioxidant indicators in the ovaries and serum of mice, including total antioxidant capacity (Pâ¯=â¯0.0004 and Pâ¯=â¯0.0032, respectively), catalase (Pâ¯=â¯0.0173 and Pâ¯=â¯0.0103, respectively) and glutathione (both P < 0.0001) were significantly increased. The oxidation indicator malondialdehyde decreased significantly (all P < 0.01). Apoptosis of ovarian granulosa cells was significantly reduced (Pâ¯=â¯0.0140) as was the expression of senescence-related proteins p53, p21 and p16 (all P < 0.01). The level of autophagy in ovarian tissue of mice treated with high increased (significantly increased LC3 protein [P < 0.0001] and significantly reduced p62 protein [Pâ¯=â¯0.0007]).CONCLUSIONS:
HNG inhibited D-gal-induced oxidative stress, apoptosis and ovarian damage, promoting ovarian autophagy. HNG may be a potential prophylactic agent against POI.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Insuficiência Ovariana Primária
/
Peptídeos e Proteínas de Sinalização Intracelular
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Reprod Biomed Online
/
Reprod. biomed. online
/
Reproductive biomedicine online
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2024
Tipo de documento:
Article