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Urinary Angiotensinogen Displays Sexual Dimorphism in Non-Diabetic Humans and Mice with Overweight.
Gonzalez, Alexis A; Visniauskas, Bruna; Reverte, Virginia; Sure, Ventaka N; Vallotton, Zoe; Torres, Bryan S; Acosta, Marco A; Zemedkun, Mahlet; Katakam, Prasad V; Prieto, Minolfa C.
Afiliação
  • Gonzalez AA; Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso 2340025, Chile.
  • Visniauskas B; Department of Physiology and Hypertension Core, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Reverte V; Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Sure VN; Department of Physiology and Hypertension Core, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Vallotton Z; Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Torres BS; Department of Physiology and Hypertension Core, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Acosta MA; Department of Physiology and Hypertension Core, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Zemedkun M; Department of Physiology and Hypertension Core, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Katakam PV; Department of Physiology and Hypertension Core, Tulane University School of Medicine, New Orleans, LA 70112, USA.
  • Prieto MC; Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112, USA.
Int J Mol Sci ; 25(1)2024 Jan 04.
Article em En | MEDLINE | ID: mdl-38203807
ABSTRACT
Increased body weight (BW) induces inappropriate renin-angiotensin system (RAS) activation. The activation of the intrarenal RAS is associated with increased urinary angiotensinogen (uAGT), blood pressure (BP), and kidney damage. Here, we examined uAGT excretion levels in young non-diabetic human subjects with overweight (OW) and non-diabetic mice with high-fat diet (HFD)-induced OW. Human subjects (women and men; 20-28 years old) included two groups (a) overweight (OW, n = 17, BMI ≥ 25); and (b) controls (normal weight (NW; n = 26, BMI ≤ 25). In these subjects, we measured BP, albuminuria, and protein levels of uAGT by ELISA adjusted by urinary creatinine (expressed by uAGT/uCrea). Mice (female and male C57BL/6J mice, 8 ± 2 weeks of age) also included two groups HFD or normal fat diet (NFD) fed for 8 weeks. We measured BW, fasting blood glucose (FBG), BP by telemetry, albuminuria, and uAGT by ELISA. In humans (i) no significant changes were observed in BP, albuminuria, and FBG when comparing NW and OW subjects; (ii) multivariate logistic regression analysis of independent predictors related to uAGT/uCrea levels demonstrated a strong association between uAGT and overweight; (iii) urinary reactive oxygen species (ROS) were augmented in men and women with OW; (iv) the uAGT/uCrea ratio was higher in men with OW. However, the uAGT/uCrea values were lower in women even with OW. In mice (i) males fed an HFD for 8 weeks became OW while females did not; (ii) no changes were observed either in FBG, BP, or albuminuria; (iii) kidney ROS were augmented in OW male mice after 28 weeks but not in females; (iv) OW male mice showed augmented excretion of uAGT but this was undetectable in females fed either NFD or HFD. In humans and mice who are OW, the urinary excretion of AGT differs between males and females and overcomes overt albuminuria.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistema Renina-Angiotensina / Angiotensinogênio / Caracteres Sexuais / Sobrepeso Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistema Renina-Angiotensina / Angiotensinogênio / Caracteres Sexuais / Sobrepeso Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile