Single-cell RNA sequencing comparison of the human metastatic prostate spine tumor microenvironment.
STAR Protoc
; 5(1): 102805, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38341849
ABSTRACT
Spinal column tumors can be difficult to process for single-cell omic studies, given the heterogeneity in tissue. Here, we present a protocol for operating room-to-benchtop single-cell processing of clinical specimens from a prostate cancer patient. We describe steps for sample homogenization, red blood cell lysis, cryopreservation, and single-cell sequencing analysis. This protocol can be used to identify prognostic markers and therapeutic targets for patients with osseous spine metastases and better inform eligibility for clinical trials.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Próstata
/
Neoplasias da Próstata
Tipo de estudo:
Guideline
Limite:
Humans
/
Male
Idioma:
En
Revista:
STAR Protoc
/
STAR protocols
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos