Applying mechanical forces on Drosophila tissues in vivo using the StretchCo, a 3D-printable device.
STAR Protoc
; 5(1): 102851, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38354083
ABSTRACT
Applying mechanical forces to tissues helps to understand morphogenesis and homeostasis. Additionally, recording the dynamics of living tissues under mechanical constraints is needed to explore tissue biomechanics. Here, we present a protocol to 3D-print a StretchCo device and use it to apply uniaxial mechanical stress on the Drosophila pupal dorsal thorax epithelium. We describe steps for 3D printing, polydimethylsiloxane (PDMS) strip cutting, and glue preparation. We detail procedures for PDMS strip mounting, tissue compaction, and live imaging upon force application. For additional details on the use and execution of this protocol, please refer to Cachoux et al. (2023)1 from which the StretchCo machine has been derived.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dimetilpolisiloxanos
/
Drosophila
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
/
STAR protocols
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
França