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Overexpression of GmPAP4 Enhances Symbiotic Nitrogen Fixation and Seed Yield in Soybean under Phosphorus-Deficient Condition.
Sun, Xi; Zhang, Huantao; Yang, Zhanwu; Xing, Xinzhu; Fu, Zhao; Li, Xihuan; Kong, Youbin; Li, Wenlong; Du, Hui; Zhang, Caiying.
Afiliação
  • Sun X; State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China.
  • Zhang H; North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding 071000, China.
  • Yang Z; State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China.
  • Xing X; North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding 071000, China.
  • Fu Z; State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China.
  • Li X; North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding 071000, China.
  • Kong Y; State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China.
  • Li W; North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding 071000, China.
  • Du H; State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China.
  • Zhang C; North China Key Laboratory for Crop Germplasm Resources of Education Ministry, College of Agronomy, Hebei Agricultural University, Baoding 071000, China.
Int J Mol Sci ; 25(7)2024 Mar 25.
Article em En | MEDLINE | ID: mdl-38612461
ABSTRACT
Legume crops establish symbiosis with nitrogen-fixing rhizobia for biological nitrogen fixation (BNF), a process that provides a prominent natural nitrogen source in agroecosystems; and efficient nodulation and nitrogen fixation processes require a large amount of phosphorus (P). Here, a role of GmPAP4, a nodule-localized purple acid phosphatase, in BNF and seed yield was functionally characterized in whole transgenic soybean (Glycine max) plants under a P-limited condition. GmPAP4 was specifically expressed in the infection zones of soybean nodules and its expression was greatly induced in low P stress. Altered expression of GmPAP4 significantly affected soybean nodulation, BNF, and yield under the P-deficient condition. Nodule number, nodule fresh weight, nodule nitrogenase, APase activities, and nodule total P content were significantly increased in GmPAP4 overexpression (OE) lines. Structural characteristics revealed by toluidine blue staining showed that overexpression of GmPAP4 resulted in a larger infection area than wild-type (WT) control. Moreover, the plant biomass and N and P content of shoot and root in GmPAP4 OE lines were also greatly improved, resulting in increased soybean yield in the P-deficient condition. Taken together, our results demonstrated that GmPAP4, a purple acid phosphatase, increased P utilization efficiency in nodules under a P-deficient condition and, subsequently, enhanced symbiotic BNF and seed yield of soybean.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Fixação de Nitrogênio Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Fixação de Nitrogênio Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China