Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities.
CRISPR J
; 7(2): 120-130, 2024 04.
Article
em En
| MEDLINE
| ID: mdl-38635326
ABSTRACT
CRISPR-Cas systems have proven effective in a variety of applications due to their ease of use and relatively high editing efficiency. Yet, any individual CRISPR-Cas system has inherent limitations, necessitating a diversity of RNA-guided nucleases to suit applications with distinct needs. We searched through metagenomic sequences to identify RNA-guided nucleases and found enzymes from diverse CRISPR-Cas types and subtypes, the most promising of which we developed into gene-editing platforms. Based on prior annotations of the metagenomic sequences, we establish the likely taxa and sampling locations where Class 2 CRISPR-Cas systems active in eukaryotes may be found. The newly discovered systems show robust capabilities as gene editors and base editors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistemas CRISPR-Cas
/
Edição de Genes
Idioma:
En
Revista:
CRISPR J
/
CRISPR j. (Online)
/
The CRISPR journal (Online)
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos