Red-Light-Induced Genetic System for Control of Extracellular Electron Transfer.
ACS Synth Biol
; 13(5): 1467-1476, 2024 05 17.
Article
em En
| MEDLINE
| ID: mdl-38696739
ABSTRACT
Optogenetics is a powerful tool for spatiotemporal control of gene expression. Several light-inducible gene regulators have been developed to function in bacteria, and these regulatory circuits have been ported to new host strains. Here, we developed and adapted a red-light-inducible transcription factor for Shewanella oneidensis. This regulatory circuit is based on the iLight optogenetic system, which controls gene expression using red light. A thermodynamic model and promoter engineering were used to adapt this system to achieve differential gene expression in light and dark conditions within a S. oneidensis host strain. We further improved the iLight optogenetic system by adding a repressor to invert the genetic circuit and activate gene expression under red light illumination. The inverted iLight genetic circuit was used to control extracellular electron transfer within S. oneidensis. The ability to use both red- and blue-light-induced optogenetic circuits simultaneously was also demonstrated. Our work expands the synthetic biology capabilities in S. oneidensis, which could facilitate future advances in applications with electrogenic bacteria.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regiões Promotoras Genéticas
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Shewanella
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Optogenética
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Luz
Idioma:
En
Revista:
ACS Synth Biol
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ACS synth. biol
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ACS synthetic biology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos