Altering Cell Junctional Tension in Spheroids through E-Cadherin Engagement Modulation.
ACS Appl Bio Mater
; 7(6): 3766-3776, 2024 Jun 17.
Article
em En
| MEDLINE
| ID: mdl-38729097
ABSTRACT
Cadherin-mediated tension at adherens junctions (AJs) is fundamental for cell-cell adhesion and maintaining epithelial integrity. Despite the importance of manipulating AJs to dissect cell-cell interactions, existing three-dimensional (3D) multicellular models have not adequately addressed the precise manipulation of these junctions. To fill this gap, we introduce E-cadherin-modified tension gauge tethers (TGTs) at the junctions within spheroids. The system enables both quantification and modulation of junctional tension with specific DNA triggers. Using rupture-induced fluorescence, we successfully measure mechanical forces in 3D spheroids. Furthermore, mechanically strong TGTs can maintain normal E-cadherin-mediated adhesion. Employing toehold-mediated strand displacement allowed us to disrupt E-cadherin-specific cell-cell adhesion, consequently altering intracellular tension within the spheroids. Our methodology offers a robust and precise way to manipulate cell-cell adhesion and intracellular mechanics in spheroid models.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Caderinas
/
Adesão Celular
/
Esferoides Celulares
Limite:
Humans
Idioma:
En
Revista:
ACS Appl Bio Mater
/
ACS appl. bio mater
/
ACS applied bio materials
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Canadá