OMIP-103: A 35-marker imaging mass cytometry panel for the co-detection of HIV and immune cell populations in human formalin fixed paraffin embedded intestinal tissue.
Cytometry A
; 105(7): 488-492, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38747672
ABSTRACT
We introduce a 35-marker imaging mass cytometry (IMC) panel for a detailed examination of immune cell populations and HIV RNA in formalin fixed paraffin embedded (FFPE) human intestinal tissue. The panel has broad cell type coverage and particularly excels in delineating subsets of mononuclear phagocytes and T cells. Markers for key tissue structures are included, enabling identification of epithelium, blood vessels, lymphatics, and musculature. The described method for HIV RNA detection can be generalized to other low abundance RNA targets, whether endogenous or pathogen derived. As such, the panel presented here is useful for high parameter spatial mapping of intestinal immune cells and their interactions with pathogens such as HIV.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por HIV
/
Inclusão em Parafina
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Citometria por Imagem
Limite:
Humans
Idioma:
En
Revista:
Cytometry A
/
Cytometry, Part A
/
Cytometry. Part A
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Austrália