Two-dimensional high-throughput on-cell screening of immunoglobulins against broad antigen repertoires.
Commun Biol
; 7(1): 842, 2024 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-38987383
ABSTRACT
Identifying high-affinity antibodies in human serum is challenging due to extremely low number of circulating B cells specific to the desired antigens. Delays caused by a lack of information on the immunogenic proteins of viral origin hamper the development of therapeutic antibodies. We propose an efficient approach allowing for enrichment of high-affinity antibodies against pathogen proteins with simultaneous epitope mapping, even in the absence of structural information about the pathogenic immunogens. To screen therapeutic antibodies from blood of recovered donors, only pathogen transcriptome is required to design an antigen polypeptide library, representing pathogen proteins, exposed on the bacteriophage surface. We developed a two-dimensional screening approach enriching lentiviral immunoglobulin libraries from the convalescent or vaccinated donors against bacteriophage library expressing the overlapping set of polypeptides covering the spike protein of SARS-CoV-2. This platform is suitable for pathogen-specific immunoglobulin enrichment and allows high-throughput selection of therapeutic human antibodies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biblioteca de Peptídeos
/
Ensaios de Triagem em Larga Escala
/
SARS-CoV-2
/
COVID-19
Limite:
Humans
Idioma:
En
Revista:
Commun Biol
/
Commun. biolog
/
Communications biology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Federação Russa