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Specific microRNAs in stallion spermatozoa are potential biomarkers of high functionality.
Ing, Nancy H; Konganti, Kranti; Ghaffar, Noushin; Johnson, Charles D; Forrest, David W; Love, Charles C; Varner, Dickson D.
Afiliação
  • Ing NH; Department of Animal Science, Texas A&M University, College Station, Texas, USA.
  • Konganti K; Texas A&M Institute for Genome Sciences and Society, Texas A&M University, College Station, Texas, USA.
  • Ghaffar N; Texas A&M Institute for Genome Sciences and Society, Texas A&M University, College Station, Texas, USA.
  • Johnson CD; AgriLife Genomics and Bioinformatics, Texas A&M University, College Station, Texas, USA.
  • Forrest DW; Department of Animal Science, Texas A&M University, College Station, Texas, USA.
  • Love CC; Large Animal Clinical Sciences, Texas A&M University, College Station, Texas, USA.
  • Varner DD; Large Animal Clinical Sciences, Texas A&M University, College Station, Texas, USA.
Reprod Domest Anim ; 59(7): e14674, 2024 Jul.
Article em En | MEDLINE | ID: mdl-39005151
ABSTRACT
Males of some species, from horses to humans, require medical help for subfertility problems. There is an urgent need for novel molecular assays that reflect spermatozoal function. In the last 25 years, studies examined RNAs in spermatozoa as a window into gene expression during their development and, more recently, for their functions in early embryo development. In clinics, more dense spermatozoa are isolated by density gradient centrifugation before use in artificial insemination to increase pregnancy rates. The objectives of the current study were to discover and quantify the microRNAs in stallion spermatozoa and identify those with differential expression levels in more dense versus less dense spermatozoa. First, spermatozoa from seven stallions were separated into more dense and less dense populations by density gradient centrifugation. Next, small RNAs were sequenced from each of the 14 RNA samples. We identified 287 different mature microRNAs within the 11,824,720 total mature miRNA reads from stallion spermatozoa. The most prevalent was miR-10a/b-5p. The less dense spermatozoa had fewer mature microRNAs and more microRNA precursor sequences than more dense spermatozoa, perhaps indicating that less dense spermatozoa are less mature. Two of the most prevalent microRNAs in more dense stallion spermatozoa were predicted to target mRNAs that encode proteins that accelerate mRNA decay. Nine microRNAs were more highly expressed in more dense spermatozoa. Three of those microRNAs were predicted to target mRNAs that encode proteins involved in protein decay. Both mRNA and protein decay are very active in late spermiogenesis but not in mature spermatozoa. The identified microRNAs may be part of the mechanism to shut down those processes. The microRNAs with greater expression in more dense spermatozoa may be useful biomarkers for spermatozoa with greater functional capabilities.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Biomarcadores / MicroRNAs Limite: Animals Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Biomarcadores / MicroRNAs Limite: Animals Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos