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MiRNA-132/212 encapsulated by adipose tissue-derived exosomes worsen atherosclerosis progression.
Guo, Bei; Zhuang, Tong-Tian; Li, Chang-Chun; Li, Fuxingzi; Shan, Su-Kang; Zheng, Ming-Hui; Xu, Qiu-Shuang; Wang, Yi; Lei, Li-Min; Tang, Ke-Xin; Ouyang, Wenlu; Duan, Jia-Yue; Wu, Yun-Yun; Cao, Ye-Chi; Ullah, Muhammad Hasnain Ehsan; Zhou, Zhi-Ang; Lin, Xiao; Wu, Feng; Xu, Feng; Liao, Xiao-Bo; Yuan, Ling-Qing.
Afiliação
  • Guo B; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Zhuang TT; Department of Metabolism and Endocrinology, General Hospital of Northern Theater Command, Shenyang, 110016, China.
  • Li CC; Department of Dermatology, Air Force Hospital of Northern Theater Command, Shenyang, China.
  • Li F; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Shan SK; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Zheng MH; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Xu QS; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Wang Y; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Lei LM; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Tang KX; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Ouyang W; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Duan JY; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Wu YY; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Cao YC; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Ullah MHE; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Zhou ZA; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Lin X; Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
  • Wu F; Department of Radiology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
  • Xu F; Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
  • Liao XB; National Clinical Research Center for Metabolic Diseases, Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, 410000, China.
  • Yuan LQ; Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China. xiaoboliaoxiangya@csu.edu.cn.
Cardiovasc Diabetol ; 23(1): 331, 2024 Sep 09.
Article em En | MEDLINE | ID: mdl-39252021
ABSTRACT

BACKGROUND:

Visceral adipose tissue in individuals with obesity is an independent cardiovascular risk indicator. However, it remains unclear whether adipose tissue influences common cardiovascular diseases, such as atherosclerosis, through its secreted exosomes.

METHODS:

The exosomes secreted by adipose tissue from diet-induced obesity mice were isolated to examine their impact on the progression of atherosclerosis and the associated mechanism. Endothelial apoptosis and the proliferation and migration of vascular smooth muscle cells (VSMCs) within the atherosclerotic plaque were evaluated. Statistical significance was analyzed using GraphPad Prism 9.0 with appropriate statistical tests.

RESULTS:

We demonstrate that adipose tissue-derived exosomes (AT-EX) exacerbate atherosclerosis progression by promoting endothelial apoptosis, proliferation, and migration of VSMCs within the plaque in vivo. MicroRNA-132/212 (miR-132/212) was detected within AT-EX cargo. Mechanistically, miR-132/212-enriched AT-EX exacerbates palmitate acid-induced endothelial apoptosis via targeting G protein subunit alpha 12 and enhances platelet-derived growth factor type BB-induced VSMC proliferation and migration by targeting phosphatase and tensin homolog in vitro. Importantly, melatonin decreases exosomal miR-132/212 levels, thereby mitigating the pro-atherosclerotic impact of AT-EX.

CONCLUSION:

These data uncover the pathological mechanism by which adipose tissue-derived exosomes regulate the progression of atherosclerosis and identify miR-132/212 as potential diagnostic and therapeutic targets for atherosclerosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Movimento Celular / Apoptose / Progressão da Doença / Miócitos de Músculo Liso / MicroRNAs / Proliferação de Células / Modelos Animais de Doenças / Aterosclerose / Exossomos / Placa Aterosclerótica Idioma: En Revista: Cardiovasc Diabetol Assunto da revista: ANGIOLOGIA / CARDIOLOGIA / ENDOCRINOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Movimento Celular / Apoptose / Progressão da Doença / Miócitos de Músculo Liso / MicroRNAs / Proliferação de Células / Modelos Animais de Doenças / Aterosclerose / Exossomos / Placa Aterosclerótica Idioma: En Revista: Cardiovasc Diabetol Assunto da revista: ANGIOLOGIA / CARDIOLOGIA / ENDOCRINOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China