The M band. Studies with fluorescent antibody staining.
J Cell Biol
; 48(2): 340-7, 1971 Feb.
Article
em En
| MEDLINE
| ID: mdl-4101522
ABSTRACT
The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 micro. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunofluorescência
/
Proteínas Musculares
/
Miofibrilas
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1971
Tipo de documento:
Article