Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes.
Histochemistry
; 55(3): 235-43, 1978 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-417051
ABSTRACT
The graphical method of Lineweaver-Burk was applied to the histochemical system to obtain quantitative information on the dopa-oxidase activity in human epidermal melanocytes. In vitro experiment showed that the incubation time necessary for yielding a certain amount of dopa-melanin is related to the amount of enzyme present. In the case of histochemical experiment, the time required for the first appearance of dopa-melanin deposition in melanocytes at various substrate concentrations was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the dopa-oxidase activity at various sites of the tissue.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Catecol Oxidase
/
Monofenol Mono-Oxigenase
/
Melanócitos
Limite:
Adult
/
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Histochemistry
Ano de publicação:
1978
Tipo de documento:
Article