A RAST assay for monitoring specific IgE antibodies in the mouse.

ABSTRACT

Mouse monoclonal anti-ovalbumin IgE was isolated from serum of BALB/c mice implanted with the IgE secreting hybridoma (14-205). Purified IgE was used to immunize rabbits and for the preparation of an antiserum specific for mouse IgE. The latter was used to develop a RAST assay to replace the PCA technique in monitoring antigen-specific IgE response in the mouse.