Hertwig effect caused by UV-irradiation of sperm of Oryzias latipes (teleost) and its photoreactivation.
Mutat Res
; 69(2): 241-8, 1980 Feb.
Article
em En
| MEDLINE
| ID: mdl-7360147
ABSTRACT
When sperm of the fish Oryzias latipes were irradiated with ultraviolet light and allowed to fertilize normal eggs, the so-called "Hertwig effect" was observed, with a dose-dependent decrease in survival rate at low doses (0-27 J . m-2) but a better survival rate at higher dose ranges. Illumination with visible light after fertilization (10-70 min after insemination) showed the existence of photoreactivation (PR), demonstrating that pyrimidine dimers are a lesion in sperm DNA that is mainly responsible for the UV-caused Hertwig effect. Genetic analysis, in which sperm from a wild-type of this fish was used, showed that, after UV-irradiation at the high dose range, male nuclei did not participate in embryonic development (a gynogenetic haploid condition). Embryos having only a maternal set of chromosomes could develop no further than stage 27. Only the visible light during the early part (until around 20-30 min after insemination, at 25 degrees C) of the single-cell stage was effective for PR; illumination thereafter was not.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Reparo do DNA
/
Peixes
/
Luz
Limite:
Animals
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
1980
Tipo de documento:
Article