Human adrenal CYP11B1: localization by in situ-hybridization and functional expression in cell cultures.
Endocr Res
; 21(1-2): 425-35, 1995.
Article
em En
| MEDLINE
| ID: mdl-7588406
ABSTRACT
CYP11B1 was detected in the human adrenal cortex and in human adenomas by in situ-hybridization methods. Specific riboprobes were generated and hybridized to sections of an Aldosterone Producing Adenoma (APA), the non-tumour portion of the corresponding adrenal gland and two adenomas not related to hyperaldosteronism. P45011B1 mRNA was clearly localized in the zona fasciculata/reticularis. Semi-quantitative analysis has been performed and seems to be applicable for a further classification of adrenal tumours. Stable expression of CYP11B1 cDNA was performed in V79 cells. The interference of different substances (metyrapone, spironolactone and different imidazole derivatives) with CYP11B1 activity was studied using this cell line. The cell line revealed to be suitable for analysis of the active site of CYP11B1 as well as for analysis of side effects of drugs on steroidogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroide 11-beta-Hidroxilase
/
Adenoma
/
Córtex Suprarrenal
/
Sistema Enzimático do Citocromo P-450
Limite:
Humans
Idioma:
En
Revista:
Endocr Res
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Alemanha