Clastogenicity of isoamylene oxide to rat lymphocytes in culture.
Mutat Res
; 347(1): 9-12, 1995 Jun.
Article
em En
| MEDLINE
| ID: mdl-7596369
ABSTRACT
The mutagenic activity of the aliphatic epoxide isoamylene oxide (2-methyl-2,3-epoxybutane) is not readily detectable in the standard Ames test. In this study, the clastogenic potential of isoamylene oxide was evaluated using an in vitro mammalian cell culture system. Approximately 48 h after establishing primary cultures of rat lymphocyte cultures, the cells were treated for 4 h with various concentrations of isoamylene oxide (50, 166.7, 500,, 1666.7, and 5000 micrograms/ml in the initial assay and 500, 1000, 2000, 3000, 4000, and 5000 micrograms/ml in the confirmatory assay). The cultures were harvested 24 h after termination of the treatment. Based upon the mitotic indices, cultures treated with the three highest concentrations in both the initial and confirmatory assays were evaluated to estimate the chromosomal aberration frequencies. Isoamylene oxide demonstrated a strong clastogenic activity in this assay up to 29% aberrant cells (without gaps) were observed at the highest concentration analyzed. The presence of an external metabolic activation system (S9) did not seem to influence the magnitude of the response at the dose levels analyzed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos de Epóxi
/
Mutagênicos
Limite:
Animals
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos