Phosphoinositide signaling in nuclei of Friend cells: tiazofurin down-regulates phospholipase C beta 1.
Cancer Res
; 55(14): 2978-80, 1995 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-7606713
ABSTRACT
Previous investigations have demonstrated the presence of conventional lipid kinases and phospholipase C (PLC) activities in nuclei of Friend erythroleukemia cells. Moreover, when Friend erythroleukemia cells are treated for 96 h with the antitumor drug tiazofurin, the induction of erythroid differentiation is accompanied by changes in amounts of both phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate due to the inhibition of an uncharacterized nuclear PLC activity. Here, we show that the nuclear PLC beta 1 isoform is down-regulated by tiazofurin (5 microM) treatment of Friend erythroleukemia cells as shown by both Western blot and Northern blot analyses for PLC beta 1 message. This indicates that PLC beta 1 down-regulation is tightly linked with erythroid differentiation of Friend erythroleukemia cells and that the autonomous nuclear signaling via inositol lipid cycle can be controlled by the antitumor drug tiazofurin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilinositóis
/
Fosfolipases Tipo C
/
Ribavirina
/
Transdução de Sinais
/
Leucemia Eritroblástica Aguda
/
Regulação para Baixo
/
Vírus da Leucemia Murina de Friend
/
Isoenzimas
Limite:
Animals
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália