The effect of frusemide on oxytocin-induced contractions of the rat myometrium.
Exp Physiol
; 79(5): 661-7, 1994 Sep.
Article
em En
| MEDLINE
| ID: mdl-7818857
ABSTRACT
Oxytocin-induced contractions of isolated strips of oestradiol-treated rat myometrium were found to be affected by exposure to the diuretic frusemide. At a concentration of 20 microM. frusemide transiently increased the force of contraction over a period of approximately 10 min. After this time there was a progressive fall in contractile force. At a higher concentration of 200 microM, only the progressive fall in force was seen until contractions were completely abolished. Frusemide has been reported to increase the activity of cAMP-phosphodiesterase in tissue extracts from oestradiol-treated rat myometrium. Therefore, the changes in contraction due to exposure to frusemide may be a reflection of the changes in intracellular cAMP resulting from a stimulation of cAMP-phosphodiesterase activity. In support of this idea, addition of dibutyryl cAMP was found to partially restore contractions after frusemide treatment. These data suggest that frusemide may be a useful tool in the manipulation of tissue cAMP levels in order to determine the different roles of cAMP in the oestradiol-treated rat myometrium.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Contração Uterina
/
Ocitocina
/
Furosemida
/
Miométrio
Limite:
Animals
Idioma:
En
Revista:
Exp Physiol
Assunto da revista:
FISIOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Federação Russa