Expanding the scope of RNA catalysis.
Science
; 264(5167): 1924-7, 1994 Jun 24.
Article
em En
| MEDLINE
| ID: mdl-8009223
ABSTRACT
The basic notions of transition state theory have been exploited in the past to generate highly selective catalysts from the vast library of antibody molecules in the immune system. These same ideas were used to isolate an RNA molecule, from a large library of RNAs, that catalyzes the isomerization of a bridged biphenyl. The RNA-catalyzed reaction displays Michaelis-Menten kinetics with a catalytic rate constant (kcat) of 2.8 x 10(-5) per minute and a Michaelis constant (Km) of 542 microM; the reaction is competitively inhibited by the planar transition state analog with an inhibition constant (Ki) value of approximately 7 microM. This approach may provide a general strategy for expanding the scope of RNA catalysis beyond those reactions in which the substrates are nucleic acids or nucleic acid derivatives.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Catalítico
Idioma:
En
Revista:
Science
Ano de publicação:
1994
Tipo de documento:
Article