Glutamate-induced increases in intracellular free Mg2+ in cultured cortical neurons.
Neuron
; 11(4): 751-7, 1993 Oct.
Article
em En
| MEDLINE
| ID: mdl-8104432
ABSTRACT
Intracellular free Mg2+ concentrations ([Mg2+]i) in single rat brain neurons were measured with the Mg(2+)-sensitive fluorescent dye magfura-2. Addition of glutamate with glycine raised [Mg2+]i from 1 to more than 11 mM compared with the resting concentration of 0.5 mM, an effect mediated by N-methyl-D-aspartate receptors. Most of the increase in [Mg2+]i was independent of extracellular Mg2+, but was dependent on extracellular Ca2+. The second component of the increase induced by glutamate was independent of extracellular Ca2+, but required extracellular Mg2+ and was amplified by extracellular Na+ removal. These results indicate that regulation of [Mg2+]i by neurotransmitters such as glutamate may be important in controlling neuronal excitability.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Córtex Cerebral
/
Receptores de N-Metil-D-Aspartato
/
Glutamatos
/
Magnésio
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Neuron
Assunto da revista:
NEUROLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
França