Clinical evidence of efficient tumor targeting based on single-chain Fv antibody selected from a combinatorial library.
Nat Med
; 2(9): 979-84, 1996 Sep.
Article
em En
| MEDLINE
| ID: mdl-8782454
ABSTRACT
We present a system for cancer targeting based on single-chain Fv (scFv) antibodies selected from combinatorial libraries, produced in bacteria and purified by using an engineered tag. Combinatorial libraries of scFv genes contain great diversity, and scFv antibodies with characteristics optimized for a particular task can be selected from them using filamentous bacteriophage. We illustrate the benefits of this system by imaging patients with carcinoembryonic antigen (CEA)-producing cancers using an iodine-123 labeled scFv anti-CEA selected for high affinity. All known tumor deposits were located, and advantages over current imaging technology are illustrated. ScFvs are produced in a cloned form and can be readily engineered to have localizing and therapeutic functions that will be applicable in cancer and other diseases.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Fragmentos de Imunoglobulinas
/
Neoplasias Colorretais
/
Antígeno Carcinoembrionário
/
Anticorpos Antineoplásicos
Limite:
Adult
/
Aged
/
Humans
/
Middle aged
Idioma:
En
Revista:
Nat Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
MEDICINA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Reino Unido